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dc.contributor.authorSantos, Wadson de Menezes-
dc.date.accessioned2026-08-07T13:21:17Z-
dc.date.available2026-08-07T13:21:17Z-
dc.date.issued2021-08-30-
dc.identifier.citationSANTOS, Wadson de Menezes. Emissões de N2O na cultura da soja inoculada com diferentes estirpes de Bradyrhizobium spp. 2021. 81f. Tese (Doutorado em Agronomia - Ciência do Solo) - Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2021.pt_BR
dc.identifier.urihttp://rima.ufrrj.br/jspui/handle/20.500.14407/25960-
dc.description.abstractA soja (Glycine max [L.] Merr.) devido ao seu teor de proteína nos grãos, necessita de elevado aporte de nitrogênio (N), que é fornecido pela fixação biológica de nitrogênio (FBN) pela inoculação de estirpes de Bradyrhizobium spp. recomendadas para soja no Brasil. Essas estirpes de bactérias possuem capacidade de desnitrificação diferentes entre si, o que pode afetar as emissões de N2O. Conhecer essa característica de cada uma das estirpes mais utilizadas no mercado pode se tornar uma estratégia para mitigar as emissões de N2O na cultura da soja. Este trabalho teve como objetivos avaliar a eficiência agronômica e as emissões de N2O na cultura da soja pela inoculação de estirpes de Bradyrhizobium spp. com diferentes capacidades desnitrificantes. Foi realizado um experimento in vitro, dois experimentos em campo, o primeiro entre os anos de 2017 e 2018, e o segundo em 2020 na Estação Experimental da Embrapa Agrobiologia, um outro experimento em casa de vegetação foi realizado entre 2019 e 2020, todos na Embrapa Agrobiologia, no município de Seropédica, RJ. O delineamento experimental foi em blocos ao acaso, com cinco repetições, e os tratamentos consistiram na inoculação das sementes de soja com estirpes de Bradyrhizobium spp. recomendadas no Brasil, que são a CPAC 7 (Bradyrhizobium diazoefficiens), CPAC 15 (Bradyrhizobium japonicum), 29 W e SEMIA 587 (Bradyrhizobium elkanii), mais o tratamento Controle (sem bactéria) e Solo (sem planta). No experimento in vitro o delineamento foi o inteiramente casualizado, com cinco repetições. Nos experimentos em campo, a estirpe 29W apresentou em geral elevada massa seca de nódulos, altas taxas de FBN e balanço positivo de N para o solo. A SEMIA 587 aumentou a produtividade em relação ao Controle, não diferindo das demais estirpes no experimento 2017/2018, já no experimento 2020 não houve efeito (p<0,05) da inoculação com Bradyrhizobium spp. O N é acumulado em maiores proporções nos grãos da soja, e as folhas senescentes e os nódulos são as partes da planta que mais contribuem com N para o solo. Nos experimentos em campo não houve efeitos das estirpes nas emissões de N2O, sendo o preparo do solo e a decomposição dos resíduos no final do ciclo e pós-colheita os fatores que mais influenciaram as emissões. Os fatores de emissão (FE) foram menores que o valor de 1% estabelecido pelo Painel Intergovernamental de Mudanças Climáticas (IPCC), exceto para o tratamento Controle e a CPAC 15 no experimento de campo 2020. Em condições de casa de vegetação a CPAC 7, SEMIA 587 e 29W apresentaram elevada FBN, e a CPAC 7 reduz as emissões de N2O na cultura da soja, resultado que foram comprovados com os genes de desnitrificação observados nos nódulos. No experimento in vitro o acetileno inibiu a óxido nitroso redutase (nosZ), permitindo uma maior emissão de N2O.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro - FAPERJpt_BR
dc.languageporpt_BR
dc.publisherUniversidade Federal Rural do Rio de Janeiropt_BR
dc.subjectÓxido nitroso redutasept_BR
dc.subject15Npt_BR
dc.subjectFixação biológica de nitrogêniopt_BR
dc.subjectNitrous oxide reductasept_BR
dc.subjectBiological nitrogen fixationpt_BR
dc.titleEmissões de N2O na cultura da soja inoculada com diferentes estirpes de Bradyrhizobium spppt_BR
dc.title.alternativeN2O emissions in soybean culture inoculated with different strains of Bradyrhizobium sppen
dc.typeTesept_BR
dc.description.abstractOtherSoybean (Glycine max [L.] Merr.) is the main Brazilian agricultural crop. Due to its high protein content (40%), it needs a high supply of nitrogen (N), which is provided by biological nitrogen fixation (BNF) by inoculating with it strains of Bradyrhizobium spp. recommended for soybeans in Brazil. These strains have different denitrification capacities that can affect N2O emissions. The management of these strains can become a strategy to mitigate N2O emissions the production of soybean. This study aimed to evaluate agronomic efficiency and N2O emissions in soybean by inoculation of strains of Bradyrhizobium spp. with different denitrifying capacities. An in vitro experiment was carried out, two field experiments were conducted, the first between the years 2017 and 2018, and the second in 2020 at the Embrapa Agrobiologia Experimental Station, another greenhouse experiment was carried out between 2019 and 2020 at Embrapa Agrobiologia, both located Seropédica, Rio de Janeiro. The experimental design was a randomized block, with five replications, and the treatments consisted of inoculating soybean seeds with strains of Bradyrhizobium sp. recommended in Brazil, which are CPAC 7 (Bradyrhizobium diazoefficiens), CPAC 15 (Bradyrhizobium japonicum), 29 W and SEMIA 587 (Bradyrhizobium elkanii), more Control (without bacteria) and Soil (without plant) treatment. In the in vitro experiment, the design was completely randomized, with five replications. In field experiments, strain 29W generally presented a high nodule dry mass, these results were also reflected in high BNF rates and positive N balance for the soil. SEMIA 587 increased productivity compared to Control, not differing from the other strains in the 2017/2018 experiment, whereas in the 2020 experiment there was no effect (P<0.05) of the inoculation with Bradyrhizobium spp. N is accumulated in greater proportions in soybeans, and senescent leaves and nodules are the parts of the plant that most contribute N to the soil. In the field experiments, there was no effect of the strains on N2O emissions, with soil preparation in the field and in the pot and the decomposition of residues at the end of the cycle and post-harvest are the factors that most influenced the emissions. EFs were less than 1% established by the Intergovernmental Panel on Climate Change (IPCC), except for Control treatment and CPAC 15 in the 2020 field experiment. Under greenhouse conditions, CPAC 7, SEMIA 587 and 29W presented high BNF, and CPAC 7 reduces N2O emissions in soybean, results that were proven with the denitrification genes observed in the nodules. In the in vitro experiment, acetylene inhibited nitrous oxide reductase (nosZ), allowing a greater emission of N2O.en
dc.contributor.advisor1Alves, Bruno José Rodrigues-
dc.contributor.advisor1IDhttps://orcid.org/0000-0002-5356-4032pt_BR
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/5238072607952859pt_BR
dc.contributor.advisor-co1Caballero, Segundo Sacramento Urquiaga-
dc.contributor.advisor-co1IDhttps://orcid.org/0000-0002-3601-1233pt_BR
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/0525790556695433pt_BR
dc.contributor.referee1Araújo, Adelson Paulo de-
dc.contributor.referee1IDhttps://orcid.org/0000-0002-4106-6175pt_BR
dc.contributor.referee1Latteshttp://lattes.cnpq.br/5394022232015318pt_BR
dc.contributor.referee2Sant'Anna, Selenobaldo Alexinaldo Cabral de-
dc.contributor.referee2IDhttps://orcid.org/0000-0001-8893-7671pt_BR
dc.contributor.referee2Latteshttp://lattes.cnpq.br/4011554123832368pt_BR
dc.contributor.referee3Madari, Beata Emoke-
dc.contributor.referee3IDhttps://orcid.org/0000-0002-6242-6619pt_BR
dc.contributor.referee3Latteshttp://lattes.cnpq.br/0302689027695014pt_BR
dc.contributor.referee4Alves, Bruno José Rodrigues-
dc.contributor.referee4IDhttps://orcid.org/0000-0002-5356-4032pt_BR
dc.contributor.referee4Latteshttp://lattes.cnpq.br/5238072607952859pt_BR
dc.contributor.referee5Jantalia, Cláudia Pozzi-
dc.contributor.referee5IDhttps://orcid.org/0000-0002-6178-6145pt_BR
dc.contributor.referee5Latteshttp://lattes.cnpq.br/3465919209024196pt_BR
dc.creator.IDhttps://orcid.org/0000-0002-0462-2275pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/3109986691043032pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Agronomiapt_BR
dc.publisher.initialsUFRRJpt_BR
dc.publisher.programPrograma de Pós-Graduação em Agronomia - Ciência do Solopt_BR
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